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Summary Attributions Wiki
XB-GRNA-23659392
gRNA Name: irx3 gRNA1
Synonyms: trop irx3 T2
Genomic Target: irx3

PAM Sequence: AGG
Sequence: 5' AAAGAACCCATATCCGACCAAGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 22/23 22/23
genome
gRNA
 
5' CCTTGGTCGGATATGGGTTCTTT 3'
   |||||||||||||||||||||||
3' GGAACCAGCCTATACCCAAGAAA 5'
 
5' CCTTGGTCGGATAGGGGTTCTTT 3'
   ||||||||||||| |||||||||
3' GGAACCAGCCTATACCCAAGAAA 5'
 
5' CCTTGGTCGGATACGGGTTCTTT 3'
   ||||||||||||| |||||||||
3' GGAACCAGCCTATACCCAAGAAA 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr4L:33777169..33777191 22/23 Sense target irx3.L
Chr4S:24713021..24713043 22/23 Sense target irx3.S
Chr6S:57745155..57745177 21/23 Sense off-target irx1.S
Chr6L:71979776..71979798 21/23 Sense off-target irx1.L
Chr9_10S:1096104..1096126 20/23 unknown
Chr1S:21531259..21531281 19/23 unknown
tropicalis 10.0 Chr4:41853106..41853128 23/23 Sense target irx3
Publications
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