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XB-PHENO-21723

Xla Wt + Mmu.ascl1S62A,S88A,S185A,S189A,S202A,S218A, Wylie LA et al. (2015)

Experiment + Assay: Xla Wt + Mmu.ascl1S62A,S88A,S185A,S189A,S202A,S218A + NF18-19 (in situ hybridization)
Background: Xla.Inbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(Mmu.ascl1S62A,S88A,S185A,S189A,S202A,S218A) ascl1.L
ascl1.S
 NF stage 2 (2-cell) blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 18 to NF stage 19  
Expression Phenotypes (2):
increased amount phox2a.S expression in anterior placodal area
mislocalised phox2a.S expression in whole organism
Source: Fig.6.a,b
Article: XB-ART-50246, PubMed