XB-LINE-999
Line Name: Xla.Tg(hsp70:dkk1-GFP;tdTomato)Tamur
| Summary |
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| Synonym: |
hsp70:Dkk1GFP, gCry:tdTomato |
| Species: |
Xenopus laevis |
| Background Strain: |
Xla.Inbred
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| Description: |
30 bp of promoter of the X. laevis hsp70 gene driving expression Dkk1GFP and 2.2 kb gamma crystallin promoter driving tdTomato. Dkk1 (labeled withGFP) specifically blocks Wnt/beta-catenin signaling and Dkk1GFP expression can be induced at a specific time-point by a heat-shock in this transgenic Xenopus. Thus, you can block Wnt/beta-catenin signaling temporally at desired time-point by a heat-shock. Transgenic X. laevis embryos were generated by a modified REMI technique using oocyte extract instead of egg extract. |
| Phenotype Description: |
dkk1-GFP expression induced temporally via heat shock, and red fluorescence in lens. |
| Color Morph: |
pigmented |
| Breeding Type: |
UNKNOWN
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| Lab of Origin: |
Tamura Lab |
| Line Type: |
Transgenic |
| Mutated Gene(s): |
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| MTA Required: |
No
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| Public: |
Yes
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| Catalog Number: |
EXRC line 79#, waiting for EXRC RRID |
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Map of the heat-shock-inducible Dkk1GFP transgene with lens labeling. The bright RFP variant, tdTomato under control of the 2.2-kb γâcrystallin promoter, was introduced downstream of hsp70-Dkk1GFP.
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Map of the heat-shock-inducible Dkk1GFP transgene with lens labeling. The bright RFP variant, tdTomato under control of the 2.2-kb γ–crystallin promoter, was introduced downstream of hsp70-Dkk1GFP.
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hsDkk1 inhibited the forelimb bud regeneration in tadpoles. (A and B) Dorsal view of the left forelimb bud at stage 54. A paddle-shaped forelimb bud can be seen in a cavity dorsal to the posterior portion of the gill region (A). The hsDkk1GFP expression was induced in the entire tadpole body, including the forelimb bud region, by heat-shock (B).
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| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
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No availability, contact the Lab of Origin for information about this Xenopus line or strain. Articles using these frogs are listed on the Attribution Tab.
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