Figure 3. Analysis of the proteins bound to plasmid beads during incubation in Xenopus egg extracts. (A) pBluescript-coupled beads were incubated for 30 min in the mock-depleted (lane 1), the ORC-depleted extracts (lanes 2) or the ORC-depleted extracts supplemented with PEG-B fraction (lane 3). (B) pG5λ6.6-coupled beads or the beads alone (no DNA) were incubated in LSS for the indicated time periods in the absence or presence of 13 μg/ml GST-p21. After incubation, the proteins bound to the beads were analyzed by western blotting with the appropriate antibodies as indicated.
Image published in: Zembutsu A and Waga S (2006)
© 2006 The Author(s). Creative Commons Attribution-NonCommercial license
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