Figure 2. . All-or-nothing nature of glutamate-induced rectification in the inner cavity. (A) Currents in response to a voltage ramp from −100 to +100 mV are shown from inside-out membrane patches excised from Xenopus oocytes in the presence and absence of 100 μM spermine. Data from WT and mutant channels, as indicated, are normalized to current at −100 mV. (B) K1/2(0) and zδ for spermine block of WT and mutant channels, obtained from I–V relationships as in A (current in spermine/control current, fitted with single Boltzmann functions). White circles denote WT and mutant channels with zδ < 1 and K1/2(0) > 1 mM. (C) Cartoon illustrating approximate physical location of the residues in the inner cavity and selectivity filter that induce strong rectification.
Image published in: Kurata HT et al. (2004)
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