Figure 5. Negative charges at the D172 position, but not E224/E299, are required for the polyamine-induced stabilization of channel open state. (A) Current traces recorded from E224G/E299S mutant channels showing neomycin (Neo) sensitivities in the presence or absence of 100 μM DA10. (B) Normalized current (I/I0)–concentration relationships for neomycin in the E224G/E299S, in the absence (ctl) and presence of 100 μM DA10. Numbers of patches and mean IC50 are indicated in the legends. (C and D) Same as A and B, except for D172N mutant channels.
Image published in: Xie LH et al. (2005)
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