Figure 6. Inhibition of Kv4.1-wild type and Zn2+ site mutants by internal MTSET. Mutant channels were expressed as described in Fig. 4 legend. (A, C, E, and G) Time-dependent inhibition of wild type, C11xA, C12xA, C13xA, and C14xA (Fig. 2 for mutant nomenclature) by internally applied MTSET (arrow, 200 μM). The y axis is the normalized peak current (peak current after MTSET/peak current before MTSET). These experiments were conducted as explained in Fig. 1 legend. The solid black line through the data points corresponds to the best-fit exponential. The second-order rate constants are indicated within the corresponding panels. (I) When all internal cysteines were mutated to alanines (C14xA), there was no inhibition by MTSET. Red symbols and gray bars represent the mean ± SEM from the number of independent measurements indicated in the corresponding panels. (B, D, F, H, and J) Representative current traces (corresponding to the left panels in each row) evoked by a step depolarization from −100 to +80 mV (inside-out patch configuration). The traces are averages (∼7–20 traces) taken before (black) and after (red) approaching the steady-state level of the inhibition.
Image published in: Wang G et al. (2005)
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