Figure 3. Quality controls of PASP. RNAs containing exons 6A or 6B were mixed in the indicated proportions (6B%) to a total amount of 1, 10 and 100 ng and reverse-transcribed, except for lanes 4, 8 and 12 where the reverse transcriptase was omitted. The resulting cDNAs were PCR-amplified for 25 cycles in a total volume of 50 µl. Five microlitres of the PCR products were electrophorised on a native agarose gel in the presence of Syber-Green and the gel was photographed. The remaining PCR products were submitted to PASP with the same dispensation order as in Figure 2D. QCs 1 and 2 were calculated as described in text and the values are given below each lane.
Image published in: Méreau A et al. (2009)
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