Fig. 7. Western blot analysis. Ten micrograms of parasite material from asexual blood stages, ookinete-enriched culture and mosquito midguts and salivary glands stages of PbGFPCON (lane 1) and pbht-gfp line (lane 2) was subjected to SDS-PAGE (10% acrylamide), transferred to a nitrocellulose membrane and probed with 1:1000 diluted anti-GFP antibody (Roche) and 1:3000 diluted HRP-conjugated anti-mouse antibody. *Ookinete-enriched culture obtained by incubation of blood sample of an infected mouse in the ookinete medium overnight at 19°C. p.i., post infection.
Image published in: Slavic K et al. (2010)
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