Figure 2. Excess Ac45 causes enhanced intragranular acidification in transgenic melanotrope cells. (A) Immunogold labeling of ultrathin sections of DAMP-incubated wild-type (wt) or Ac45-transgenic (tg) Xenopus melanotrope cells with the anti-DNP antibody in combination with 10-nm protein-A gold. Gold labeling was predominantly found in the secretory granules (arrows) and lysosomes (L). Bars equal 200 nm. (B) Quantification of intragranular DAMP accumulation. The number of gold particles per μm2 found in 205 wt and 206 tg dense-core secretory granules of 20 randomly selected wt or tg melanotrope cells from three wt or tg frogs.
Image published in: Jansen EJ et al. (2010)
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