Figure 5. mMCPH1 can be converted into a humanlike, competent form by specific amino acid substitutions. (A) Schematic representation of the constructs used in B. The N-terminal domain of hMCPH1 was divided into two subdomains, Ah (amino acids 1–145) and Bh (amino acids 146–195), and the corresponding domain of mMCPH1 was divided into Am (amino acids 1–151) and Bm (amino acids 152–194), accordingly. A chimera form (AmBh) was created, and single or double point mutations (T72R and E142K) were further introduced in an attempt to confer a humanlike activity on the mouse sequence (mouse to human). (B) Mouse to human conversion. The constructs shown in A were subjected to the cell-free assay. (C) Human to mouse conversion. Two point mutations (R66T and K136E) were introduced singly or doubly into the human sequence, and the resulting constructs were subjected to the cell-free assay. cond, condensin; N-ter., N-terminal; XG, XCAP-G; XH2, XCAP-H2.
Image published in: Yamashita D et al. (2011)
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