Figure 3. DPP6K markedly represses the recovery-accelerating effects of KChIP3a.Representative current traces generated during the two-pulse protocol used to measure recovery from inactivation for Kv4.2+KChIP3a (A), Kv4.2+KChIP3a+DPP6a (B), Kv4.2+KChIP3a+DPP6S (C), and Kv4.2+KChIP3a+DPP6K (D). A 1-sec depolarization to +50 mV was delivered to maximally inactivate the channels, followed by an increasing recovery interval at −100 mV before applying a 250-ms test pulse at +50 mV to check the degree of recovery from inactivation. (E) Fractional recovery was plotted as a function of the interval duration at −100 mV. The residual value at the end the first pulse was subtracted from the peak current values of the first and second pulses, and the fractional recovery was determined by dividing the peak value of the second pulse by that of the first pulse.
Image published in: Jerng HH and Pfaffinger PJ (2012)
Jerng, Pfaffinger. Creative Commons Attribution license
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