Fig. 6. Overview of 5mC-immunostaining of axolotl LBCs. a In the portion of a single LBC bivalent shown here a predominantly chromomeric staining pattern that parallels the DAPI staining intensity is apparent under low magnification. The bulk of the loop chromatin, as indicated by pol II (mAb H5) immunostaining appears not to show general 5mC immunostaining. b Regions of centromeric heterochromatin are reflected by the appearance of loopless chromatin bars (brackets)—the examples here are close to the HLB loci (arrowheads) on LBC6. The intensity of 5mC in the heterochromatin appears similar to that of the DAPI staining, as in flanking chromomeres, suggesting that 5mC is not enriched in lampbrush heterochromatin. Note some laterally projecting fibrils of 5mC staining are apparent in neighbouring regions even at this low magnification (two examples indicated by arrows)
Image published in: Morgan GT et al. (2012)
Image downloaded from an Open Access article in PubMed Central. © The Author(s) 2012
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