Figure 5. Deletion of RIM15 or IGO1 and IGO2 affects the positive feedback loop for Cdk1 activation.A–B. Serial dilutions of strains with the indicated genotypes were spotted on YEPD plates and incubated for 48 hours at the indicated temperatures. C. Cycling (cyc) cultures of wild type, igo1Δ igo2Δ and igo1Δ igo2Δ swe1Δ cells were arrested in G1 by α-factor and released in fresh medium at 38°C. At the indicated time points cells were collected for FACS analysis of DNA contents (not shown), kinetics of budding, spindle assembly/elongation and nuclear division (graphs), as well as to make protein extracts for western blot analysis of Clb2 and Cdc5 levels. Pgk1 was used as loading control. D. Serial dilutions of strains with the indicated genotypes were spotted on YPD and YPGal plates and incubated at 25°C for 2 days.
Image published in: Juanes MA et al. (2013)
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