Fig. 2. Animal cap assay showing dependence of Msx and other neural and neural plate gene expression on canonical Wnt and modulated BMP signaling. Ectoderm was isolated from mid/late blastula stage embryos (St. 7-8) injected at the one-cell stage with 300 pg of Wnt3a RNA and variable amounts of chordin RNA, ranging from zero to 5 ng, cultured to early neurula (St. 14) and processed for Northern blot analysis using methylmercury hydroxide agarose gel electrophoresis. Note that compared to the other NC genes AP2a, Slug and Sox9, Msx genes are up-regulated significantly by Wnt3a alone and also are not fully extinguished by the highest dose of chordin, which activates neural plate genes such as Sox2. UI, uninjected animal caps; W, whole embryo. Ethidium bromide staining of 18S ribosomal RNA is shown to control for equal RNA loading.
Image published in: Khadka D et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher.
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