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FIGURE 8 The glossopharyngeal and vagal nerves. Whole mount antibody staining of the nerves (anti-acetylated alpha-tubulin) and muscles (anti-desmin) of a NF stage 47/48 X. laevis tadpole. (a and b) 3D depth coding views of the nerves. (a) dorsal view of the region of the otic capsule. (b) dorsal view of the distal branchial region. (c–l) maximum intensity projections of the same digital stack. Nerves are shown in green, muscles are shown in red. (c) dorsal overview of head and branchial region. (d) ventral overview of the head and branchial region. (e–l) magnifications of the areas marked by the strippled rectangles in (c) and (d). The white asterisks indicate the innervation sites of the branchial muscles by rami/ramuli of the glossopharyngeal (IX) and vagal nerves (X). The yellow arrows indicate the route of the ramulus constrictor arcus branchiarium III of the X. (l) dorsal view of the cucullaris muscle. The white scale bar in (c) and (d) is 200 mm, in (e) to (l)50 mm [Color figure can be viewed at wileyonlinelibrary.com]

Image published in: Naumann B and Olsson L (2018)

Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons.

GeneSynonymsSpeciesStage(s)Tissue
tubal3.Ltuba1X. laevisSometime during NF stage 47 to NF stage 48nervous system
head
cranial nerve
glossopharyngeal nerve
vagus nerve

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