Fig. 1. Developmental expression profiles of idh1 and idh2 revealed through RT-PCR and WISH. (A) RT-PCR analysis showing stage-specific expression of idh1 and idh2. Ornithine decarboxylase (odc) was used as a loading control. (B) Semi-quantitative analysis of RTPCR results. Band intensities were measured using ImageJ, and the intensity of each gene at the indicated developmental stages was normalized to the corresponding odc band. Normalized values were plotted to compare the stage-specific expression dynamics of idh1 and idh2. (C) Whole-mount in situ hybridization (WISH) showing idh1 and idh2 expression in the craniofacial region, somites, and pronephric kidney, including the IT and distal dPT. atp1a1 was used as an anatomical reference to identify pronephric tubule regions. Scale bars: 500 um. RT-PCR, reverse transcriptionpolymerase chain reaction; WISH, whole-mount in situ hybridization; s, somites; PT, proximal tubule; IT, intermediate tubule; dPT, distal pronephric tubule.
Image published in: Lee HK et al. (2026)
Copyright © 2026. Image reproduced with permission of the Publisher.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| idh2.L | X. laevis | Throughout NF stage 33 and 34 | pronephric kidney head somite late proximal tubule pronephric duct early distal tubule | |
| atp1a1.L | atp1a1-a, atp1a1-b, K-ATPase, Na+/K+ ATPase, Na+K+ ATPase, Na+K+ATPase | X. laevis | Throughout NF stage 35 and 36 | pronephric kidney proximal tubule pronephric duct early distal tubule otic vesicle brain spinal cord ventral posterior hindgut endoderm |
| idh1.L | X. laevis | Throughout NF stage 35 and 36 | pronephric kidney head somite proctodeum late proximal tubule pronephric duct early distal tubule |
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