Fig. 2. RA signaling inhibits lens regeneration, and RA signaling antagonism does not inhibit lens regeneration. (A) Liarozole and TTNPB are potent inhibitors of cornea-lens regeneration. A high concentration of RA very strongly inhibits lens regeneration. ⁎ indicates p<0.0001, using two-tailed Fischer's exact test. (B) A drug-treatment validation assay using qPCR shows that all of the drugs strongly activate RA signaling, as seen by a profound upregulation of cyp26a1, a marker of active RA signaling, in the cornea. ⁎ indicates p<0.01, using unpaired t test. (C) Citral and LE-135, inhibitors of RA signaling, fail to inhibit cornea-lens regeneration. (D) A drug-treatment validation assay using qPCR shows that LE-135 attenuates expression of cyp26a1 in the cornea. ⁎ indicates p<0.0001, using unpaired t test. All error bars indicate standard error.
Image published in: Thomas AG and Henry JJ (2014)
Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V.
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